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. 2017 Sep 8;10(5):433–450. doi: 10.1007/s12195-017-0508-5

Figure 6.

Figure 6

Both MEK and p38 are necessary for stimulation-induced DUSP6 expression in myoblasts. (a, b) Select phosphoprotein time-courses for inhibitor conditions. n = 3 replicates are plotted as mean ± SEM. p values from ANOVA comparisons over the full time-course. (a) Phospho-MEK1 for Control, FEI, and FEI + p38i; (b) Phospho-ERK1/2 for Control, TI, and TI + p38i conditions; (c, d) DUSP1 and DUSP6 expression by RT-qPCR in myoblast following either FEI or TI with either PD0325901 (MEKi), SB203580 (p38i) or 0.1% DMSO-only (–) co-treatment. p values from Student’s t tests or not significant (n.s.); (e) DUSP6-based feedback model.