Figure 1.

(a, b, c) Schematics showing dimensions of a single maze, a single microfluidic device, and an array of the devices inside of a stage-top custom incubation chamber, respectively. (d) Phase contrast microscopy image of a single maze network. Scale bar is 100 µm. (e) Photograph of a single microfluidic device, consisting of 20 maze networks that share a central chemoattractant reservoir. A circular 3 mm diameter hole was punched in the center of each device in order to deposit the PDGF-BB into the reservoir. Cells were seeded outside of the devices. Scale bar is 1 mm. (f) An array of microfluidic devices placed inside a stage-top custom incubation chamber, consisting of two PDMS blocks bonded to the top of a glass slide. Scale bar is 5 mm.