Figure 2.

(a) Experiment setup schematic, showing the custom incubation chamber used for culturing on the automated microscope’s stage. The setup consisted of a benchtop variable power supply, which provided a current of 0.5 A to an ITO glass that was placed on top of the PDMS chamber. Heat generated by the glass warmed up the media inside of the chamber. Temperature was measured using a RTD sensor coupled to a PID controller, which modulated the power supply in order to maintain a constant temperature of 37 °C. (b) Photograph of the incubation chamber in operation. Electric current was fed to the resistive layer of the ITO glass through a pair of copper electrodes. This led to heat generation underneath of the PDMS chamber. The incubation chamber was placed on top of a XY motorized stage, controlled by the computer, for imaging.