Figure 2.

MTT Assay evaluation of inhibitor efficiency of (a) NaN₃/DOG at 37 °C, (b) chlorpromazine, (c) dynasore and (d) nystatin for different CNP formulations (5% v/v) for fold-increase calculations. A non-synchronized population of HCC-1806 cells (10,000) was plated in 96 well plates for 24 h before performing the treatments. Cells were first incubated with inhibitors for 1 h followed by CNP formulation addition. Experiments were performed in triplicates. Fold Increase greater than or equal to 1.30 was considered significant.