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EC release of laminin during dynamic co-culture. (a–d) EC monolayers (phalloidin red, DAPI blue) dynamically stimulated for 3 days in transwell co-culture systems secrete laminin (green) into the extracellular space. Laminin penetrates through the 10 μm thick membrane as indicated by (>). Laminin is also present on the membrane as indicated by arrowheads. Z-stack height 23 μm. Scale bar 50 μm (a, b, d) or 25 μm (c).
