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. 2017 Aug 2;10(5):463–481. doi: 10.1007/s12195-017-0498-3

Figure 1.

Figure 1

Doxorubicin is less cytotoxic to breast cancer cells cultured in fibroblast-conditioned media or co-culture. (a) Schematic of conditioned media experiments: conditioned media is harvested from incubated HDFs (Fbs) after 24 h and applied to MDA-MB-231 breast tumor cells (TCs). (b) Live TCs assessed by nuclear dead stain ± doxorubicin (10 μM) in Fb-conditioned or control media after 24 h as percent of total TCs (n = 5). (c) Cellular uptake of doxorubicin by TCs at successive time points after Doxorubicin (10 μM) application as assessed by fluorescent signal of lysed cells (n = 6). (d) Schematic of insert co-culture experiments: MDA-MB-231 cells (TCs) and HDFs (Fbs) are co-cultured independent of contact for 24 h prior to doxorubicin treatment. In the Fb-conditioned experimental group, the Fbs are removed prior to dosing chemotherapy. (e) Live TCs assessed by nuclear dead stain ± doxorubicin (10 μM) in Fb-conditioned or control media after 24 h as percent of total TCs (n = 5). (f) Cellular uptake of doxorubicin by TCs at 6 h after Doxorubicin (10 μM) application as assessed by fluorescent signal of lysed cells (n = 6). Data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 by paired t-tests (b, e, f) and two-way ANOVA followed by post hoc paired t-tests (c).