Skip to main content
. 2017 Aug 2;10(5):463–481. doi: 10.1007/s12195-017-0498-3

Figure 3.

Figure 3

Ratiometric response of tumor cells occurs when cells are co-cultured in a breast-mimetic collagen I matrix. (a) Schematic of independent tissue culture insert set-ups for increasing ratios of TCs:Fbs in a collagen I matrix. Arrow indicates direction of application of doxorubicin for 18 h. (b) Fluorescent images of Celltracker deep red-labeled MDA-MB-231(red, TCs) and total cell nuclei (blue, NucBlue) in 3D collagen hydrogels prior to doxorubicin treatment. Cells were seeded at varied ratios with overall cellular concentration held constant (1E6 cells/mL). Blue label without red indicates a fibroblast. (c) Live TCs assessed by nuclear dead stain within collagen gels ± doxorubicin (10 μM) after 18 h application via interstitial flow (n = 3). Data are represented as mean ± SEM. *p < 0.01, ***p < 0.001 by post hoc unpaired t-tests following two-way ANOVA analysis. ***Signifies statistical significance to matched + doxorubicin condition.