Figure 4.
Agent-based model (ABM) predicts overall and distance-dependent response of tumor cells to doxorubicin in the 3D collagen hydrogel system. (a) Diffusion-convection concentration gradient developed in Comsol within a simulated 3D hydrogel. Scale gives normalized concentration (C/Cmax). (b) The agent-based model incorporates concentration profiles, cancer cells, and fibroblasts for in silico drug screening. (c) ABM-predicted overall viability for homogenous gels of varied tumor cell:fibroblast ratios (n = 20). (d) Location dependent viability of tumor cells in homogenous gels seeded at varied tumor cell:fibroblast ratios (n = 20). Data are represented as mean ± SEM. ****p < 0.0001 compared to 231 control by post hoc unpaired t-tests following two-way ANOVA analysis. (d) post hoc t-tests after MANOVA analysis which shows significant effects (p < 0.001) for ratios and distance