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. 2016 Nov 24;31(5):e22098. doi: 10.1002/jcla.22098

Table 1.

PCR amplification conditions

Genetic variant Primer sequences Annealing temperature PCR fragment
NAT2*5C‐T341C 5′‐ TGGTGTCTCCAGGTCAATCA‐3′ 60°C 361 bp
5′‐ GGTGTTTCTTCTTTGGCAGG‐3′
NAT2*6B‐G590A 5′‐GGACCAAATCAGGAGAGAGCAG‐3′ 60°C 159 bp
5′‐GTTGGAGACGTCTGCAGGTATG‐3′
NAT2*7B‐G857A 5′‐GCTGGGTCTGGAAGCTCCTC‐3′ 62°C 547 bp
NAT2*18‐A845C 5′‐TTGGGTGATACATACACAAGGG‐3′
NAT2*5A‐C481T 5′‐AAGGATCAGCCTCAGGTGCCTT‐ 3′ 60°C 84 bp
5′‐ CTGCTCTCTCCTGATTTGGTCC‐3′