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Enzymatic reaction has as principle the activation of reaction between sodium‐dependent β‐D‐galactosidase and its substrate O‐nitrophenyl‐β‐D‐galactopyranose (ONPG) by converting it to O‐nitrophenyl (ONP) and galactose by Na+ present in the sample resulting in the formation of the yellow (λmax 405 nm) compound ONP
