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The enzymatic reaction for K+ assay is based on conversion of phosphoenolpyruvate into pyruvate by K+ dependent pyruvate kinase. In presence of reduced nicotinamide adenine dinucleotide (NADH), the pyruvate generated is converted to lactate in a reaction catalyzed by lactate dehydrogenase (LDH). The K+ concentration present in the sample is proportional to reduction of the optical density at 380 nm as a function of NADH oxidation to nicotinamide adenine oxidized dinucleotide (NAD +)
