Table 1.

Primer Sequence

	Primer Name	Primer Sequence	Reference	Region of HCV
First round	Sc2	GGGAGGTCTCGTAGACCGTGCACCATG	Ohno et al. 23	Core
First round	Ac2	GAG(AC)GG(GT)AT(AG)TACCCCATGAG(AG)TCGGC
Sequencing	S7	AGACCGTGCACCATGAGCAC
Sequencing	A5	TACGCCGGGGGTCA(TG)T(GA)GGGCCCCA
	Mix X
Second round	S7	AGACCGTGCACCATGAGCAC
Second round	S2a	AACACTAACCGTCGCCCACAA
Second round	G1b	CCTGCCCTCGGGTTGGCTA(AG)
Second round	G2a	CACGTGGCTGGGATCGCTCC
Second round	G2b	GGCCCCAATTAGGACGAGAC
Second round	G3b	CGCTCGGAAGTCTTACGTAC
	Mix Y
Second round	S7	AGACCGTGCACCATGAGCAC
Second round	G1a	GGATAGGCTGACGTCTACCT
Second round	G3a	GCCCAGGACCGGCCTTCGCT
Second round	G4	CCCGGGAACTTAACGTCCAT
Second round	G5a	GAACCTCGGGGGGAGAGCAA
Second round	G6a	GGTCATTGGGGCCCCAATGT
First round	P1203	GGGTTCTCGTATGATACCCGCTGCTTTGACTC	Harris et al. 40	NS5b
First and Second round	P1204	GGAGGGGCGGAATACCTGGTCATAGCCTCCGTGAA
Second round	NS5b IP	TGATACCCGCTGCTTTGACTCNACNGTCAC
First round	GOP1	AGCGTCTAGCCATGGCGT	Harris et al. 40	5′NCR
First round	GOP2	GCACGGTCTACGAGACCT
Second round	GIP1	GTGGTCTGCGGAACCGG
Second round	GIP2	GGGCACTCGCAAGCACCC