Table 2.
Repeatability of unlabeled probe HRM
| Genotype | Within‐run | Between‐run | ||
|---|---|---|---|---|
| Homozygotes | Tm (SD), °C | CV, % | Tm (SD), °C | CV, % |
| 778WT | 76.36 (0.11) | 0.04 | 76.44 (0.06) | 0.07 |
| p.R778L | 72.14 (0.05) | 0.06 | 72.19 (0.12) | 0.17 |
| 992WT | 82.06 (0.09) | 0.11 | 81.98 (0.21) | 0.26 |
| p.P992L | 77.25 (0.09) | 0.12 | 77.34 (0.23) | 0.17 |
| 1106WT | 71.09 (0.06) | 0.08 | 71.21 (0.21) | 0.29 |
| p.V1106I | 66.47 (0.05) | 0.08 | 66.60 (0.18) | 0.27 |
| Heterozygotes | ΔTm (SD), °C | CV, % | ΔTm (SD), °C | CV, % |
|---|---|---|---|---|
| 778WT/p.R778L | 4.47 (0.29) | 1.96 | 4.57 (0.15) | 3.28 |
| 992WT/p.P992L | 4.57 (0.08) | 1.75 | 4.70 (0.17) | 3.61 |
| 1106WT/p.V1106I | 4.73 (0.04) | 0.85 | 4.86 (0.13) | 4.73 |
The probe melting temperatures (Tm) from within‐run and between‐run showing averaged Tms, difference between melting temperatures (ΔTm) for heterozygotes, standard deviations (SD, in parentheses), and coefficient of variation (CV) are listed.