Figure 1.

Typical layout of each chip and the designs of the subarrays. A, Layout of 1 agarose‐modified glass slide containing 16 physically isolated subarrays. B, B and A were one‐to‐one correspondence, and Samples 1‐16 were the serum samples to be tested, with SDs0‐4 as calibrator and Control as quality control serum in each chip. C, All the initial probes with 4 lines × 6 rows, including 12 antibodies (namely, anti‐HGH antibody, anti‐f‐PSA antibody, anti‐NSE antibody, anti‐CA125 antibody, anti‐Ferritin antibody, anti‐CA199 antibody, anti‐AFP antibody, anti‐CEA antibody, anti‐CA242 antibody, anti‐β‐HCG antibody, and anti‐CA153 antibody) were printed onto agarose‐modified glass slides in 2 replicates of a row and blocked, which were completed by the manufacturer before selling