Specificity assay of RT‐LAMP. M: 250 bp DNA marker. (A) Electrophoresis and dye pattern of calcein and HNB, Lanes 1‐4: Sputum samples of Mtb, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae. (B) Electrophoresis and dye pattern of calcein and HNB, Lanes 1‐9: H37Rv, Mycobacterium intracellulare, M. marinum, M, kansasii, M. avium, M. flavescens, M. smegmatis, M. fortuitum, and M. chelonae