Figure 1.

(A‐B) ARMS‐PCR and sequence of the amplified fragment in TNF‐α gene polymorphism. The genotype was labeled on corresponding sequences, and the sites which were marked were the SNP of TNF‐α gene electrophoresed on 2% agarose, stained with ethidium bromide. Left to right: M, DNA marker (100bp), Lanes 1 and 2: GG homozygous (323bp and 224 bp), Lane 3: homozygote AA (323bp and 154 bp) and Lanes 4 and 5: AG heterozygote (323 bp, 224 bp, and 154 bp) for TNF‐α genotype