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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: J Neurochem. 2019 Jun 18;151(2):255–272. doi: 10.1111/jnc.14711

Fig. 7. Effect of 24-h anoxia on expression of LRRC8 subunits.

Fig. 7

(a) Relative abundance of mRNA species for five members of the LRRC8 family (LRRC8A-E) in cultured rat astrocytes, quantified using qRT-PCR. Data are the mean values ± SEM normalized to the housekeeping gene ribosomal RPL13a and then LRRC8A within the same samples in four astrocyte cultures. (b) Changes in the LRRC8A-E subunit expression following 24-h anoxia normalized to expression of the same gene in control (normoxic) cells (∆∆Ct values). and validation of anoxia-induced gene expression changes in the same cells. Expression of the HIF-1 dependent gene VEGFA hypoxia/anoxia marker was quantified immediately after 24 h anoxia. Data are the mean values ± SEM from 4 experiments. *p<0.05 vs. normoxic control.