Table 2:
Precision, Accuracy and Reproducibility for detection of pyr-Val and THB-Val in human globin spiked with authentic standards.
| A: Spiked control HB [fmol/sample] | ||||
|---|---|---|---|---|
| HB spiked* | mean | SD | CV | |
| Day 1 | HB-Val | 9.25 | 1.23 | 13% |
| THB-Val | 138.23 | 19.93 | 14% | |
| pyr-Val | 11.72 | 2.35 | 20% | |
| Day 2 | HB-Val | 16.52 | 1.47 | 9% |
| THB-Val | 225.29 | 14.19 | 6% | |
| pyr-Val | 20.90 | 1.44 | 7% | |
| Day 3 | HB-Val | 12.33 | 0.36 | 3% |
| THB-Val | 203.58 | 9.62 | 5% | |
| pyr-Val | 23.94 | 1.28 | 5% | |
| Overall | HB-Val | 12.70 | 1.02 | 8% |
| THB-Val | 189.03 | 14.58 | 8% | |
| pyr-Val | 18.86 | 1.69 | 11% | |
| B: Patient globin analyzed in quintuplet [pmol/g globin] | ||||
| Patient ID | mean | SD | CV | |
| #04 | THB-Val | 2919.31 | 171.3 | 6% |
| pyr-Val | 758.09 | 26.0 | 3% | |
| #06 | THB-Val | 1157.83 | 57.47 | 5% |
| pyr-Val | 514.29 | 34.2 | 7% | |
| #08 | THB-Val | 2104.93 | 100.9 | 5% |
| pyr-Val | 882.39 | 26.0 | 3% | |
*
Globin samples (2mg) were spiked with 10, 200 and 20 fmol/sample HB-Val, pyr-Val and THB-Val, respectively, and analyzed by LC/MS/MS.