Table 3:
Amount of N-terminal valine adducts (pmol/g globin) before and after 6 days of Treosulfan treatment.
| Subject ID |
Day 0 | Day 6 | ||||
|---|---|---|---|---|---|---|
| pyr-Val | THB-Val |
pyr-Val THB-Val |
pyr-Val | THB-Val |
pyr-Val THB-Val |
|
| #01 | 0.41 | 4.61 | 0.09 | 262.4 | 169.0 | 1.55 |
| #02 | 0.15 | 4.58 | 0.03 | 193.7 | 127.2 | 1.52 |
| #03 | 0.25 | 1.72 | 0.14 | 201.3 | 138.0 | 1.46 |
| #04 | 0.21 | 4.03 | 0.05 | 385.3 | 262.4 | 1.47 |
| #05 | 0.14 | 4.38 | 0.03 | 453.1 | 316.0 | 1.43 |
| #06 | 0.23 | 5.22 | 0.04 | 171.5 | 109.0 | 1.57 |
| #08 | 0.42 | 4.08 | 0.10 | 260.9 | 217.3 | 1.20 |
| #10 | 0.25 | 5.24 | 0.05 | 136.6 | 85.1 | 1.61 |
| #13 | 0.41 | 7.95 | 0.05 | 262.7 | 215.7 | 1.22 |
| #14 | 0.58 | 5.73 | 0.10 | 221.9 | 184.4 | 1.20 |
| #07* | 0.32 | 6.65 | 0.05 | 188.4 | 260.4 | 0.72 |
| #09* | 0.22 | 5.58 | 0.04 | 256.3 | 292.9 | 0.88 |
| #11* | NA | NA | 195.2 | 291.4 | 0.67 | |
| #12* | 0.25 | 7.38 | 0.03 | 244.9 | 280.2 | 0.87 |
| mean | 0.29 | 5.17 | 245.31 | 210.63 | ||
| SD | 0.10 | 1.7 | 89.6 | 78.5 | ||
Globin was isolate and analyzed by LC/MS/MS. Day 0 samples were taken before the first dose of treosulfan and day 6 samples were taken after 6 days of treatment.
*
Samples were not included in correlation analysis because their pyr-Val/THB-Val radios at day 6 were significantly different form that of the others (Student t-test, mean±SD 1.39±.15 vs 0.77±.10, p<0.0001).