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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Tuberculosis (Edinb). 2019 Jul 30;118:101854. doi: 10.1016/j.tube.2019.07.005

Figure 6. Membrane insertion of EsxA is affected by both membrane unsaturation and charge.

Figure 6.

The NBD-labeled WT and Q5K were incubated with 800 μM of the indicated liposomes at 4 °C for 30 min in 20 mM TrisHCl and 100 mM NaCl pH 7.4 buffer. The protein-liposome mixture was transferred into a cuvette at RT. 0.1 volume of 1 M sodium acetate (pH 4) was added to drop the pH. The solution was continuously stirred while recording the data at RT. The emission spectra were recorded at 544 nm with excitation at 488 nm, and the representative spectra are shown in (A) for WT and (B) for Q5K. The fold changes relative to DOPC were shown in (C).