Table 3. Effects of membrane unsaturation and charge on membrane insertion of WT and Q5K.

The data of membrane insertion of WT and Q5K in the presence of various lipids were collected from Fig 6 and 5. Membrane insertion of WT in DOPC was set as 1.0, and all other data were compared to WT in DOPC.

Lipids	# of carbon and double bond in fatty acid tails	Unsaturation (%)	Phase Transition Temperature (°C)	Net-Charge/lipid molecule	Membrane insertion of WT	Membrane insertion of Q5K
DOPC	2x (18:1)	100	−17	0	1	0.63
DOPG	2x (18:1)	100	−18	−1	1.08	0.52
POPC	16:0-18:1	50	−2	0	0.45	0.30
POPG	16:0-18:1	50	−2	−1	1.90	0.90
POPC(4):POPG(1)	16:0-18:1	50	−2	80% neutral:20% negative	1.4	0.90
DPPC	2x (16:0)	0	41	0	ND	ND
DPPG	2x (16:0)	0	41	−1	ND	ND