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. 2019 Oct 22;10:1337. doi: 10.3389/fphys.2019.01337

Figure 4.

Figure 4

eIF4E is a direct target of Malat1/miR-150 signal axis. (A) Diagram of the miR-150 putative binding sites in eIF4E 3′-UTR. (B) Luciferase reporter assays show that miR-150 mimic transfection significantly reduces the luciferase activity of the reporter vector containing eIF4E-wt compared with mimic-NC transfection, whereas cotransfection with pcDNA-Malat1 and miR-150 mimic significantly relieves the inhibitory effect of miR-150 mimic transfection on luciferase activity. (C,D) eIF4E mRNA and protein expression in PDGF-BB-treated ASMCs transfected with mimic-NC, miR-150 mimic, miR-150 mimic + pcDNA-Malat1, si-NC, or si-Malat1 determined by RT-PCR and western blot, respectively. *p < 0.05.