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eIF4E knockdown inhibits PDGF-BB-induced ASMC proliferation and migration. ASMCs are transfected with si-NC or si-eIF4E, and then are harvested for the following detections. (A,B) eIF4E mRNA and protein expression detected by RT-PCR and western blot, respectively. (C) Cell proliferation determined by MTT assay. (D) Cell migration detected by Transwell assay (scale bar: 100 μm). (E) PCNA and MMP-9 expression determined by western blot assay. *p < 0.05.
