Figure 1.

Human macrophages are equally susceptible to both JUNV variants. Human monocyte-derived macrophages (HMDM) cells were infected at a multiplicity of infection (MOI = 1) with the attenuated C#1 or the pathogenic P strains of JUNV. (A) Morphological observation of the cells. JUNV-infected cells become more flattened and extended. (B) Infective titration of cell supernatants was performed in Vero cells for 6 days by a standard PFU method. (C) Viral antigens were detected by immunofluorescence with a pool of antibodies and anti-JUNV-FITC, and nuclei were counterstained with DAPI. (D) HMDM cells were infected with C#1 or P strains and at 3 dpi cells were stained with anti-JUNV-FITC and the percentage of viral antigen positive cells were analyzed by flow cytometry. The percentage of infected cells was compared using the Non-parametric One-way ANOVA followed by Dunn's multiple comparison test. The results are graphed as the median (min-max, horizontal line indicates the median). At least three independent donors were used in each assay.