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Amplification test for viral inactivation: Immunofluorescence images of vaccine viruses at the 3rd passage. Green cytoplasmic fluorescence is indicative of viral replication and blue fluorescence localizes to the nucleus of the infected Vero cells. Images were obtained by staining with a PEDV-specific polyclonal antibody. (A) Untreated PEDV, (B) Heat and RNAse treated. PEDV at the 3rd passage showing minimal replication, (C) Irradiated PEDV at the 3rd passage.
