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Targeted amplicon sequencing was performed on cfDNA to analyze the genes indicated, all of which are implicated in the pathogenesis of lung cancer. Exon tiling to assess the majority of the noted gene, hotspot analysis of relevant areas of genes, and copy-number variant analysis were performed as indicated. Abbreviations: cfDNA = cell-free DNA; indels = in-frame deletions; SNV = single nucleotide variation.
