Skip to main content
. 2019 Sep 16;117(8):1381–1386. doi: 10.1016/j.bpj.2019.09.006

Figure 2.

Figure 2

Unilamellarity increases with charged lipids and/or decreasing solvent ionic strength. (A) The fraction of externally accessible lipid facc (related to extent of unilamellarity) increases with an increasing abundance of charged lipid dopant (POPG or 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS)) in POPC bilayers extruded through 100-nm pores. Samples hydrated in PBS (blue circles) show a lower facc at all concentrations of charged lipid compared to hydration in pure water. Blue and orange stars represent facc calculated from cryoEM images of POPC in PBS and POPC plus PG in H2O, respectively. (B) Shown is a typical cryoEM image of POPC vesicles extruded (100 nm) in water. Scale bars, 100 nm. Arrows highlight multilamellar structures, whereas arrowheads highlight nested vesicles. (C) Distribution of structures in 100 nm vesicles extruded in water were obtained from cryoEM. To see this figure in color, go online.