Figure 2.
The Aggregate Interactome Identifies Indirect Interactions of APPSw and Tau
(A) Identified interacting partners of APP (E2 domain) in insoluble SY5Y-APPSw aggregates.
(B) Domain architecture of APP protein, as inferred from the Protein DataBank (PDB). ECM, extracellular matrix; 1mwp, 3nyl, 1iyt: APP domain IDs in PDB.
(C) Protein-protein docking of SRRM2 and the APP-E2 domain corroborates observed cross-linking data (yellow highlighted area).
(D) Protein-protein docking of TOP1 with APP-E2 corroborates observed cross-linking data (yellow highlighted oval indicates concordant evidence of interaction).
(E) Tau-interacting proteins identified from SY5Y-APPSw insoluble aggregates. Proteins in dashed boxes also interact with the APP-E2 domain.
(F) Interactions of phosphorylated tau peptides, characteristic of hP-TAU, identified in insoluble aggregates from SY5Y-APPSw. SRSF6_P (dashed box) also interacts with APP-E2.