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. 2019 Sep 9;20:25–41. doi: 10.1016/j.isci.2019.09.005

Figure 1.

Figure 1

Lgl1 Conditional Knockout in Pyramidal Neurons Led to Increased Numbers of Asymmetric Synapses

(A) Electron micrographs taken 150–200 μm (top) or 50 μm (bottom) ventral to the CA1 pyramidal neuron layer in the schaffer collateral region of P14 mice. Red arrows denote asymmetric synapses. Black arrows denote symmetric synapses. Scale bar, 500 nm. Quantification of synapse numbers corresponding to each region: N = 6 control, 6 Lgl1 cKO animals.

(B) Electron micrographs taken 150–200 μm (top) or 50 μm (bottom) ventral to the CA1 pyramidal neuron layer in the schaffer collateral region of 8-week-old mice. Red arrows denote asymmetric synapses. Black arrows denote symmetric synapses. Quantification of synapse numbers corresponding to each region: N = 5 control, 5 Lgl1 cKO animals.

(C) Representative traces of mEPSC recordings from acute slices from P13–P15 control and Lgl1 cKO mice. Quantification of mEPSC frequency and amplitude: n = 22 control, 19 Lgl1 cKO neurons.

(D) Representative traces of mIPSC recordings from acute slices from P14 control and Lgl1 cKO mice. Quantification of mIPSC frequency and amplitude: n = 20 control, 19 Lgl1 cKO neurons.

(E) Representative confocal images of oblique CA1 dendrites filled with Alexa Fluor 555 hydrazide. Scale bar, 10 μm.

(F and G) (F) Quantification of spine density and (G) distribution of spine morphology.

(H) Quantification of cumulative distributions of synapse ultrastructure measurements in P14 control and cKO animals: n = 194 control, 174 Lgl1 cKO synapses. Quantification of cumulative distributions of synapse ultrastructure measurements in 8-week old control and cKO animals: n = 194 control, 208 Lgl1 cKO synapses.

(I) Biochemical fractionation from wild-type P14 mice. P2, crude synaptosomal; SMF, synaptic membrane fraction; PSD, postsynaptic density.

(J) Representative traces of NMDAR currents and combined AMPAR/NMDAR currents from acute slices taken from P14 control and Lgl1 cKO mice following Lgl1 deletion at P7. Scale bar, 100 pA (vertical); 100 ms (horizontal). Quantification of the calculated ratio of AMPAR current to NMDAR current: n = 13 control, 13 Lgl1 cKO neurons.

*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.