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. 2019 Sep 9;20:25–41. doi: 10.1016/j.isci.2019.09.005

Figure 2.

Figure 2

Lgl1 Inhibits Synapse Formation by Inhibiting aPKC and Promotes the Expression of Vangl2

(A) Electron micrographs taken 150–200 μm (top) or 50 μm (bottom) ventral to the CA1 pyramidal neuron layer in the schaffer collateral region of P14 mice. Red arrows denote asymmetric synapses. Black arrows denote symmetric synapses. Scale bar, 500 nm. Quantification of synapse numbers corresponding to each region: N = 8 control, 5 aPKC dcKO animals.

(B) Electron micrographs taken 150–200 μm (top) or 50 μm (bottom) ventral to the CA1 pyramidal neuron layer in the schaffer collateral region of 8-week-old mice. Red arrows denote asymmetric synapses. Black arrows denote symmetric synapses. Quantification of synapse numbers corresponding to each region: N = 5 control, 8 aPKC dcKO animals.

(C) Quantification of cumulative distribution of synapse ultrastructure measurements in P14 control and cKO animals: n = 194 control, 129 aPKC dcKO synapses. Quantification of cumulative distribution of synapse ultrastructure measurements in 8-week-old control and cKO animals: n = 87 control, 168 aPKC dcKO synapses.

(D) Electron micrographs taken 150–200 μm (top) or 50 μm (bottom) ventral to the CA1 pyramidal neuron layer in the schaffer collateral region of P14 mice. Red arrows denote asymmetric synapses. Black arrows denote symmetric synapses. Scale bar, 500 nm. Quantification of synapse numbers corresponding to each region: N = 7 control, 6 Lgl1;PKCι/λ;PKCζ tcKO animals.

(E) Electron micrographs taken 150–200 μm (top) or 50 μm (bottom) ventral to the CA1 pyramidal neuron layer in the schaffer collateral region of 8-week-old mice. Red arrows denote asymmetric synapses. Black arrows denote symmetric synapses. Quantification of synapse numbers corresponding to each region: N = 8 control, 10 Lgl1;PKCι/λ;PKCζ tcKO animals.

(F) Levels of Vangl2 and JAM-C proteins in P2 fractions by Western blots.

(G) Quantification of Vangl2 and JAM-C protein levels in P2 fraction. N = 5 for Vangl2. N = 4 for JAM-C.

*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.