Figure 4.

Lgl1 Deletion at P7 Produced a Subset of SMS-like Behavioral Phenotypes

(A) Open-field analysis following P7 deletion of Lgl1 showing representative trajectories (left) and heatmaps (right) of control and Lgl1 cKO animals.

(B) Quantification of distance traveled during the test. N = 30 control, 27 Lgl1 cKO.

(C) Quantification of time spent in the outer region of the field (thigmotaxis) during the first and second 5-min periods.

(D and E) (D) Representative heatmap and quantification (E) of animal preference for objects during the sample phase of the novel object recognition (NOR) test. N = 23 control, 19 Lgl1 cKO animals.

(F and G) (F) Representative heatmaps and quantification (G) of animal preference for objects during the test phase of the NOR test. White asterisk denotes location of the novel object.

(H) Representative heatmaps from the habituation period of the social interaction (SI) test.

(I and J) (I) Quantification of time spent in and (J) preference for regions of interest (ROIs) representing future location of novel mice and objects. N = 25 control, 24 Lgl1 cKO animals.

(K) Representative heatmaps during the social interaction phase of the SI test. White asterisk denotes location of the novel mouse. Opposite chamber contains the object.

(L and M) (L) Quantification of time spent in and (M) preference for ROIs containing either the novel mouse or novel object. Positive value indicates preference for the novel mouse. N = 25 control, 22 Lgl1 cKO animals.

(N) Representative heatmaps during the social novelty phase of the SI test. White asterisk denotes location of the novel mouse. Opposite chamber contains the previously explored mouse.

(O and P) (O) Quantification of time spent interacting with and (P) preference for mice during the social novelty phase. Positive value indicates preference for the novel mouse. N = 20 control, 14 Lgl1 cKO animals. NS, nonsocial; S, social.

∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.