Figure 5.

Conditional Triple Knockout of Lgl1, PKCι/λ, and PKCζ Rescued Asymmetric Synapse Number and Cognitive Deficit

(A) Open-field analysis following P7 deletion of Lgl1 showing representative trajectories (left) and heatmaps (right) of control and aPKC dcKO animals.

(B) Quantification of distance traveled during the test. N = 32 control, 26 aPKC dcKO animals.

(C) Quantification of time spent in the outer region of the field (thigmotaxis) during the first and second 5-min periods.

(D) Representative heatmap and quantification of animal preference for objects during the sample phase of the novel object recognition (NOR) test. N = 26 control, 15 aPKC dcKO animals.

(E) Representative heatmaps and quantification of animal preference for objects during the test phase of the NOR test. White asterisk denotes location of the novel object.

(F and G) (F) Quantification of time spent in and (G) preference for regions of interest (ROIs) representing future location of novel mice and objects. N = 35 control, 26 aPKC dcKO animals.

(H and I) (H) Quantification of time spent in and (I) preference for ROIs containing either the novel mouse or novel object. Positive value indicates preference for the novel mouse. N = 35 control, 27 aPKC dcKO animals. *p < 0.05; **p < 0.01.

(J and K) (J) Quantification of time spent interacting with and (K) preference for target mice during the social novelty phase. Positive value indicates preference for the novel mouse. N = 35 control, 27 aPKC dcKO animals. *p < 0.05.

(L) Open-field analysis following P7 deletion showing representative trajectories (left) and heatmaps (right) of control and Lgl1;PKCι/λ;PKCζ tcKO animals.

(M) Quantification of distance traveled during the test. N = 20 control, 14 Lgl1;PKCι/λ;PKCζ tcKO animals.

(N and O) (N) Representative heatmap and quantification (O) of animal preference for objects during the sample phase of the novel object recognition (NOR) test. N = 21 control, 15 Lgl1;PKCι/λ;PKCζ tcKO animals.

(P and Q) (P) Representative heatmaps and quantification (Q) of animal preference for objects during the recognition test phase of the NOR test. White asterisk denotes location of the novel object.

(R and S) (R) Quantification of time spent in and (S) preference for ROIs representing future location of novel mice and objects.

(T and U) (T) Quantification of time spent in and (U) preference for ROIs containing either the novel mouse or novel object. Positive value indicates preference for the novel mouse.

(V and W) (V) Quantification of time spent interacting with and (W) preference for target mice during the social novelty phase. Positive value indicates preference for the novel mouse. N = 25 control, 19 Lgl1;PKCι/λ;PKCζ tcKO animals. NS, nonsocial; S, social.

*p < 0.05; **p < 0.01; ***p < 0.001.