Figure 5.

Loading dynamics of local actin fluorescence intensity enhancement at the point loading site of HeLa cells upon microneedle indentation assay. (A₁)−(A₆) denote the serial images of actin (green) fluorescent intensity in 1 min with 3D reconstruction every 10 s (thus, six frames in total). The needle was in hover at the first frame (first 10 s) as in (A₁) and (B₁) and then loaded on the top of the nucleus (blue) at the second frame (10–20 s) as in (A₂) and (B₂). Dashed square boxes in (A₁)−(A₆) depicted the region of local actin intensity enhancement as magnified in inserts (the region with local actin intensity enhancement was embraced by an irregular dashed contour, the central hole of which indicated the site occupied by the needle). (C) Spatiotemporal distribution of RFI along the white dotted line in dashed boxes in (A₁₋₆) is shown. (D) Evolution of mean RFI ± SD in the entire dashed regions in inserts is shown. Asterisks showed significant difference among two to six frames to the first one. ^∗∗∗∗p < 0.0001. n = 19 from two repeats. To see this figure in color, go online.