Figure 6.

Neutrophils were applied to the top of a bead-containing 0.3% agarose gel. The gel plus neutrophils were imaged on the confocal microscope immediately after neutrophils were added, 1 h later, and 2 h later. The number of beads visible in each frame (solid symbols) were counted to determine where the gel top was located because the liquid medium was free of beads. Neutrophils (hollow symbols) were counted for the frames where their midplanes were in focus. Dashed lines connecting neutrophil counts are a guide to the eye. To see this figure in color, go online.