Figure 4.

Spatiotemporal Mapping of the Expression of Genes Encoding Podocyte-Expressed Factors and Predicted Endothelial and Interstitial Cell Interactions

(A–E) (A) In situ hybridization for FBLN2, NTN4, and BMP4 indicating gene expression (arrowheads) during intermediate phases of proximal fate to podocyte development. Arrowheads point to expression in developing podocytes that is absent in mature RCs, with the exception of NTN4, which is expressed in periglomerular vasculature and at lower levels in mature podocytes. Immunostaining in SSB and RC for (B) FBLN2, (C) HSPG2, (D) NTN4, (E) UNC5B, and indicated markers.

(F–H) (F) In situ hybridization of UNC5B. Phospho-SMAD1/5- (G) and phospho-ERK- (H) positive cells (arrowheads) showing labeling of infiltrating cells into the glomerular cleft in SSB and in RCs (scale bars, 25 μm). Dist, Distal Nephron; Med, Medial Nephron; Prox, Proximal Nephron; Pod, Podocytes; PE, Parietal Epithelium; SSB, S-Shaped Body; RC, Renal Corpuscle.

(I) Dot pots showing relative expression (blue color intensity) and relative number of positive nuclei (dot size) for extracellular matrix and cell signaling genes found within clusters identified in Figure 1A.

See also Figure S5.