Figure 6.

Histology and Gene Expression in the BAT of Orexin-Deficient Mice

(A) Representative images of BAT stained with hematoxylin and eosin from newborn wild-type (WT) and orexin-deficient (ORXKO) mice. Scale bars, 40 μm.

(B) Lipid droplet areas of the BAT in newborn WT and ORXKO mice (12 mice per group).

(C) Expression of orexin receptor genes in BAT from newborn wild-type mice (n = 7). The expressed is presented relative to the mean of orexin receptor expression in the brain.

(D) Western blot images of UCP1 and β-tubulin in BAT of newborn WT and ORXKO mice.

(E) Expression of genes related to the function and differentiation of BAT in newborn WT mice (n = 11) and ORXKO (n = 10). The expression is presented relative to the mean of newborn WT mice. **p < 0.01 obtained using t test with Bonferroni correction.

(F) Representative images of BAT stained with hematoxylin and eosin from adult mice. Scale bars, 40 μm.

(G) Adipocyte numbers of the BAT in adult male WT and ORXKO mice fed normal chow or on HFD (12 mice for each group).

(H) Expression of orexin receptor genes in BAT from adult wild-type mice fed normal chow (n = 6, 4–6 months old). The expression is presented relative to the mean orexin receptor expression in the brain. Ox2r was not detected in BAT.

(I) Representative western blot images of UCP1 and β-tubulin in BAT of chow-fed 14-week-old WT and ORXKO mice.

(J) Expression of genes related to the function and differentiation of BAT in adult male WT and ORXKO mice, which were fed normal chow or HFD (n = 10 for each group).

The expression is presented relative to the mean of WT mice fed normal chow. *p < 0.05, **p < 0.01, ***p < 0.001. All data are presented as mean ± SEM. See also Figure S2.