Figure 1.

A population of CD8 Primary Effectors Expressing the Chemokine Receptor CXCR5 Is Generated Shortly after rLm-OVA Infection

Naive wild-type mice received 10⁴ CD45.1⁺ OT-1 cells and were infected 2 days later with 2 × 10⁴ colony-forming unit of rLm-OVA.

(A–C) The frequency of OT-1 cells among CD8⁺ T cells in the spleen (A), the rLm-OVA burden in the spleen and the liver (B), and the frequency of Tfh among CD4⁺ T cells in the spleen (C) at various time points after infection. The data are from three to five independent experiments with at least three mice per time point.

(D) The intensity of CXCR5 expression by OT-1 and non-OT-1 CD8⁺ T cells, expressed as mean fluorescence intensity (MFI). Statistical significance of differences between OT-1 and non-OT-1 cells is indicated (Wilcoxon test).

(E) The percentage of CXCR5⁺ cells among OT-1 cells. Representative plots are also shown.

(F) The intensity of CXCR5 expression (as corrected geometric MFI, i.e., [MFI^OT-1 – MFI^non-OT-1]) by CXCR5⁺ and CXCR5^- OT-1 cells.

(G) The percentages of CXCR5⁺ cells (red) and CXCR5^- cells (blue) among OT-1 cells, total CD8⁺ T cells, and total CD3⁺ T cells. Statistical significance is indicated (Wilcoxon test).

(H) The geometric MFI of CD44, KLRG-1, PD-1, CD127, IL-21R, CD40, and CD62L expression by CXCR5⁺ OT-1 (red), CXCR5^- OT-1 (blue), and non-OT-1 CD8⁺ T cells (black) on day 4 after infection. Representative plots are also shown. The data are from three independent experiments with at least three mice per time point. Friedman's test was used for statistical comparison. p.i., post-infection. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.