Figure 7.

Lifetime and position of GlpG intermediates observed when using a soft spring for 40 simulations. (A) The number of trajectories as a function of lifetime between the first unfolding event and the fully extended state for the 20 unfolding trajectories originating from either end of GlpG are shown. (B) The corresponding aggregate time spent at each extension in an intermediate is shown. Each peak represents an intermediate (one is observed for the N→C pathway and two for the C→N pathway). The fully unfolded state has an extension above 50 nm, which defines the upper limit of the x axis. To see this figure in color, go online.