FIG 10.

miR-7 levels are elevated in brains of obese mice (hypothalamus) and in Alzheimer’s disease patients. (A) qRT-PCR analysis of pre-miR-7a-1 and mature miR-7-5p in the hypothalamus of mice fed a chow diet (CD) or DIO mice (n = 5 per group). *, P < 0.05 compared with CD mice. (B) qRT-PCR analysis of pre-miR-7a-1 and mature miR-7-5p in the hypothalamus of mice fed a chow diet (CD) or a HFD (n = 5 per group). (C and D, top) Representative Western blot analysis measuring levels of Aβ in the media of N2a-APPsw (C) and BV-2 (D) cells after transfection with a CM or miR-7. (Bottom) Relative Aβ levels corresponding to the means ± SEM from two independent experiments performed in triplicate. *, P < 0.05 (significantly different from cells transfected with the CM [normalized to a value of 1]). (E) qRT-PCR analysis of mature miR-7-5p in human cortex and cerebellum from human AD patients and matching healthy human subjects (n = 5 per group). *, P < 0.05 compared with control healthy subjects. (F) qRT-PCR analysis of IRS-2 and IDE in cortex from human AD patients and matching healthy human subjects (n = 5 per group). *, P < 0.05 compared with control healthy subjects.