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. 2019 Jan 2;32(11):1664–1672. doi: 10.5713/ajas.18.0589

Table 1.

Characterization of the nine microsatellites loci used for short tandem repeat genotype

Group number Locus Primer (5′→3′) Repeat motif Size of alleles (bp) Annealing temperature (°C)
1 WWX1 F:ATGGATGCTAACAAACACTC (CCAAT)4 127–152 59
R:GTACAAAGGTCATGGAGAAG
CKW13 F:AGGCTGAGGTGGGAGAATTTAT (AAAC)5 150–154 53.4
R: TTCTTCCACTTCTCCCAAAGAA
2 CKW14 F:AACTGATCCGGCAGAAAACTAA (CCT)5 219–223 60
R:ACTTAGCATGCAGCTTCACAAA
CKW21 F:CCCAGAACAGTGCTAGAAGAGG (TTA)10 236–272 60
R:AGCGAGTCACTCCAGTACCTTC
G10 F:ACGCTGGCAGATCTTGATGTC (CT)7 156–170 57.1
R: TTAAAGCCTGTTCTCTGTAC
3 TTUCG5 F:GGGTGTTTTCCAACTCAG (TCTAT)8 175–305 53.7
R:CACTTTCCTTACCTCATCTT
CKW49 F:TGAACACACATGCAGACTGG (CA)10 188–208 62.1
R: TTTGCGAGACAGAGCCTTTT
4 G07 F:ACAGGTGATGCTATTATTACG (AT)12 144–172 55
R: CATTCCCTAGGAACAACCTGC
CKW32 F:CAGTGCAAGTTCACCCACAG (AAAAT) 7 155–235 55.4
R: TCGAGAGCACTCCATTTTGA