Table 1.
Group number | Locus | Primer (5′→3′) | Repeat motif | Size of alleles (bp) | Annealing temperature (°C) |
---|---|---|---|---|---|
1 | WWX1 | F:ATGGATGCTAACAAACACTC | (CCAAT)4 | 127–152 | 59 |
R:GTACAAAGGTCATGGAGAAG | |||||
CKW13 | F:AGGCTGAGGTGGGAGAATTTAT | (AAAC)5 | 150–154 | 53.4 | |
R: TTCTTCCACTTCTCCCAAAGAA | |||||
2 | CKW14 | F:AACTGATCCGGCAGAAAACTAA | (CCT)5 | 219–223 | 60 |
R:ACTTAGCATGCAGCTTCACAAA | |||||
CKW21 | F:CCCAGAACAGTGCTAGAAGAGG | (TTA)10 | 236–272 | 60 | |
R:AGCGAGTCACTCCAGTACCTTC | |||||
G10 | F:ACGCTGGCAGATCTTGATGTC | (CT)7 | 156–170 | 57.1 | |
R: TTAAAGCCTGTTCTCTGTAC | |||||
3 | TTUCG5 | F:GGGTGTTTTCCAACTCAG | (TCTAT)8 | 175–305 | 53.7 |
R:CACTTTCCTTACCTCATCTT | |||||
CKW49 | F:TGAACACACATGCAGACTGG | (CA)10 | 188–208 | 62.1 | |
R: TTTGCGAGACAGAGCCTTTT | |||||
4 | G07 | F:ACAGGTGATGCTATTATTACG | (AT)12 | 144–172 | 55 |
R: CATTCCCTAGGAACAACCTGC | |||||
CKW32 | F:CAGTGCAAGTTCACCCACAG | (AAAAT) 7 | 155–235 | 55.4 | |
R: TCGAGAGCACTCCATTTTGA |