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. 2019 Oct 28;8(11):63. doi: 10.1038/s41389-019-0172-9

Fig. 7. Effects of MOB1- and LATS1/2-knockdown by siRNA on the YAP activation promoted by PR55α.

Fig. 7

a MOB1 was knocked down by siRNAs in both HPNE and CD18/HPAF cells with/without the expression of ectopic PR55α and PR55α-shRNA, respectively. After 72 h siRNA-transfection, the cells were analyzed for the phosphorylation and/or level of MOB1, LATS1/2, and YAP by immunoblotting. GAPDH served as an internal control. The levels of YAP and GAPDH were quantified using ImageJ software. YAP protein levels were normalized by the corresponding GAPDH levels and relative YAP levels in the samples were analyzed by SigmaPlot software and presented as bar graphs. b LATS1 and/or LATS2 were knocked down by siRNAs in CD18/HPAF and AsPC-1 cells expressing Control-shRNA or PR55α-shRNA. After 72 h incubation in medium containing Dox (2 µg/ml) to maintain shRNA expression, the cells were analyzed for the phosphorylation and/or levels of PR55α, LATS1, LATS2, YAP, and GAPDH. The levels of YAP and GAPDH were quantified using ImageJ software and relative YAP protein levels versus GAPDH levels were indicated under the YAP blots