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. 2019 Oct 28;9:15389. doi: 10.1038/s41598-019-51849-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Protein size is not a substantial contributor to protein loss in non-denaturing photocapture conditions. Triplicate BMPA hydrogels with immobilized protein were created for each of OVA (42.7 kDa), TI (20.1 kDa), and RNase (13.7 kDa). Dotted lines represent photobleaching controls, whereas solid lines represent treatment groups. At round 7, the protein losses were 46.0% ± 5.0%, 38.2% ± 4.2%, and 71.4% ± 7.9% for OVA, TI, and RNase, respectively, indicating minimal size-based correlation.