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. 2019 Oct 28;9:15389. doi: 10.1038/s41598-019-51849-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of Fluorescence Loss Between Protein-Conjugate and Immunoreagents during Serial Stripping and Reprobing Cycles. (A) Fluorescence loss of protein-conjugates in three BMPA hydrogels immobilized with RNase-488 (DOL = 1.3). Round 0 corresponds to the RNase-488 fluorescence after the initial immunoprobing round. (B) Fluorescence loss of immunoreagents in BMPA hydrogels immobilized with RNase-488 and immunoprobed with primary (Rb anti-RNase) and fluorescent secondary (Gt anti-Rb conjugated to Alexa Fluor 633) antibodies. Each incubation cycle following round 0 corresponds to one round of stripping, followed by immediate reprobing with new immunoreagents.