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. 2019 Oct 28;9:15446. doi: 10.1038/s41598-019-51189-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gross liver pictures and IF of transplanted mice 2 months post-transplant. (A) Gross morphology of sham transplant mice livers and HFH transplanted mice livers. Transplanted mice livers were softer in texture and less nodular than livers of sham transplant mice. (B) Co-labelling between IF of anti human albumin and anti human alpha1-antitrypsin (A1AT), (C) CYYP2A6, (D) CYP3A4, and (E) absence of co-localization between anti human albumin and AFP. This demonstrated that the engrafted hepatocytes matured, and demonstrated structural and functional viability up to 2 months post-transplant. No immature hepatocytes were seen.