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. 2019 Oct 28;9:15401. doi: 10.1038/s41598-019-51895-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Biochemical analysis of the metagenomic FabI2 ENR. Initial velocities of the reaction were determined in duplicate as a function of varying concentrations of (a) the cofactor (NADH) and (b) substrate crotonyl-CoA. Data were fit to the Michaelis–Menten equation choosing the nonlinear regression equation using GraphPad Prism version 7. The fitted line and K_m values are shown.