Table 3.
B. breve UCC2003-fumA1-fumST1T2 genes that were transcriptionally upregulated during growth in mMRS medium supplemented with 2′-FL or lactose as the sole carbohydrate, as compared to growth in mMRS supplemented with ribose.
| Gene ID | Gene name | Function | 2′-FL | Lactose |
|---|---|---|---|---|
| Bbr_0526 | lntR | LacI transcriptional regulator | — | — |
| Bbr_0527 | lntP1 | ABC transporter permease | 2.61 | 3.81 |
| Bbr_0528 | lntP2 | ABC transporter permease | 3.35 | 2.12 |
| Bbr_0529 | lntA | GH42 Beta-galactosidase | 2.48 | 2.21 |
| Bbr_0530 | lntS | ABC transporter solute binding protein | — | — |
| Bbr_1551 | lacS | Galactoside symporter | 18.72 | 22.27 |
| Bbr_1552 | lacZ6 | GH2 Beta-galactosidase | 12.98 | 9.05 |
| Bbr_1553 | lacI | LacI transcriptional regulator | — | — |
| Bbr_1740 | fumF | L-2-keto-3-deoxy-fuconate aldolase | 4.16 | — |
| Bbr_1741 | fumD | L-fuconolactone hydrolase | 4.69 | — |
| Bbr_1742 | fumP | L-fucose permease | 4.49 | — |
| Bbr_1743 | fumC | L-keto-3-deoxy-fuconate-4-dehydrogenase | 3.70 | — |
| Bbr_1744 | fumE | L-fuconate dehydratase | 7.12 | — |
| Bbr_1745 | fumR | LacI transcriptional regulator | — | — |
The level of expression is shown as a fold-value of increase in expression on each carbohydrate, as compared to a ribose control, with a cut-off of a minimum 2.0-fold increase in expression. Microarray data were obtained using B. breve UCC2003-fumA1-fumST1T2 grown on 1% 2′-FL or lactose and were compared with array data obtained when B. breve UCC2003-fumA1-fumST1T2 was grown on ribose as a control. The cut-off point was set at 2.0-fold, with a P value of <0.001. With the - sign are indicated fold values below the cut-off. The predicted functions were determined using a combination of BLASTP71, Pfam (http://pfam.xfam.org) and KEGG (http://www.genome.jp/kegg/) searches.