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. Author manuscript; available in PMC: 2019 Oct 29.
Published in final edited form as: Cell Rep. 2019 Sep 17;28(12):3105–3119.e7. doi: 10.1016/j.celrep.2019.08.036

Figure 3. miR-21 Is Transferred to Monocytes and/or Macrophages after Uptake of Tumor-Derived EVs.

Figure 3.

(A) Monocytes and/or macrophages (MOs and/or macrophages) from miR-21-null mice were identified by FACS as CD11bhigh/CD45high cells (magenta gate). MOs and/or macrophages were then sorted on the basis of the GFP signal detected as the upper limit in control (no tumor).

(B) In mice implanted with GL261.palmGFP.H2B.mRFP, a population of GFP-positive MOs and/or macrophages was identified (green gate in the GFP/RFP plot).

(C) Uptake of EV-GFP resulted in the presence of miR-21 in MOs and/or macrophages.

(D) EV-GFP uptake was visualized by imaging flow cytometry using ImageStream. Scale bar, 10 μm.

Data represent three independent experiments and are presented as the mean with SEM (error bars). One-way ANOVA with Tukey’s multiple-comparisons test.