(A) Normalized read count of genes involved in microglia proliferation (Hammond et al., 2019).
(B) Representative images of in vitro culture miR-21-null microglia transfected with miR-21 mimic and control showing DAPI and Ki67 staining 24 h after transfection. Scale bar, 100 μm.
(C) Quantification of proliferation 24 h after transfection with miR-21 mimic or control as measured by Ki67-positive cells per total number of DAPI-positive cells.
(D) Representative images of in vitro culture miR-21-null microglia transfected with siRNA against Btg2 and siRNA control showing DAPI and Ki67 staining 24 h after transfection. Scale bar, 100 μm.
(E) Quantification of proliferation 24 h after transfection with siRNA against Btg2 or control as measured by Ki67-positive cells per total number of DAPI-positive cells.
Data represent three independent experiments and are presented as the mean with SEM (error bars). ***p < 0.001, multiple testing adjusted p value differential expression in (A), and ***p < 0.001, unpaired t test in (C), (E), and (F).