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. 2019 Oct 2;8(20):e012138. doi: 10.1161/JAHA.119.012138

Figure 7.

Figure 7

Profibrotic effect of marinobufagenin on cultured left ventricular myocytes of Dahl salt‐sensitive rats kept on a low salt (LS) diet (n=6). Addition of marinobufagenin (1 and 10 nmol/L) for 24 hours to the culture media–activated production of: (A) transforming growth factor (TGF) β‐1 (mature form); (B) Mothers Against DPP Homolog (SMAD) 2; (C) mitogen‐activated protein kinase (MAPK) 42/44; (D) collagen‐1; (E) increased amount of phosphorylated protein kinase C (PKC) δ, and (F) decreased level of Friend leukemia integration 1 transcription factor (Fli‐1). Top panels: representative Western blotting images; bottom panels: statistical analysis of band density for each protein standardized for GAPDH. Each bar represents the mean±standard error of the mean; each protein was tested at least 5 times for each condition. By 1‐way ANOVA followed by Neuman–Keuls post hoc test: *P<0.05, **P<0.01 one of the other groups vs baseline (0 nmol/L marinobufagenin); † P<0.05, 10 nmol/L vs 1 nmol/L marinobufagenin.