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. 2019 Oct 2;8(20):e012338. doi: 10.1161/JAHA.119.012338

Figure 3.

Figure 3

Blocking extracellular HSP (heat shock protein) 70 restricts doxorubicin‐induced myocardial inflammation. A through E, At the indicated time points, the protein levels of interleukin‐6, transforming growth factor (TGF)‐β1, interleukin‐17A, and interleukin‐10 in heart tissue homogenates were detected by Western blot analysis. In a time‐dependent manner, HSP70 neutralizing antibody treatment inhibited the increase in the levels of interleukin‐6 (B), TGF‐β1 (C), and interleukin‐17A (D) but upregulated the level of interleukin‐10 (E) in the myocardium. Representative immunoblots and the ratio of the indicated protein to GAPDH are presented. Data are the mean±SEM of 3 assays. # P<0.05, ## P<0.01, compared with normal mice; *P<0.05, **P<0.01, compared with doxorubicin‐treated mice. F, The expression of the proinflammatory proteins inducible NO synthase (iNOS) and cyclooxygenase 2 as well as the anti‐inflammatory protein B‐cell lymphoma 3 (BCL3) in heart tissue was examined by Western blot analysis at the experimental end point. Representative immunoblots and the ratio of the indicated protein to GAPDH are presented. Data are the mean±SEM of 3 assays. # P<0.05, compared with normal mice; *P<0.05, compared with doxorubicin‐treated mice.